Plasmid

Part:BBa_K1441012

Designed by: Derrica Burke   Group: iGEM14_Georgia_State   (2014-10-10)


DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

This part contains the vector PGAPzα A + modified Multiple Cloning Site(MCS) fixed according to iGEM standards. We replaced the MCS with DNA ligase kit plate part(BBa_K525710). PGAPzα A + Linker has α-secretion factor before the DNA ligase kit plate part, which allows yeast cells and some Eukaryotic cells to secrete the protein outside of the cell membrane after translation. Following the insert there is a MYC epitope tag and 6x histag. These are used to tag the protein so it can be easily identified by westernblot technique.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1663


[edit]
Categories
Parameters
None